Product Info Summary
SKU: | PA1791 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-LIM and SH3 domain protein 1 LASP1 Antibody
SKU/Catalog Number
PA1791
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LIM and SH3 domain protein 1 LASP1 Antibody catalog # PA1791. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LIM and SH3 domain protein 1 LASP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1791)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1, identical to the related rat sequence and different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1791 is reactive to LASP1 in Human, Mouse, Rat
Applications
PA1791 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
11 kDa
Calculated molecular weight
29.717kDa
Background of LASP1
LASP1 (LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al. (1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-LASP1 antibody, PA1791, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 3: Rat Intestine Tissue Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: A431 Cell Lysate
Lane 7: HELA Cell Lysate
Lane 8: 293T Cell Lysate
Click image to see more details
Anti-LASP1 antibody, PA1791, IHC(P)
IHC(P): Human Lung Cancer Tissue
Click image to see more details
Anti-LASP1 antibody, PA1791, ICC
ICC: A549 Cell
Protein Target Info & Infographic
Gene/Protein Information For LASP1 (Source: Uniprot.org, NCBI)
Gene Name
LASP1
Full Name
LIM and SH3 domain protein 1
Weight
29.717kDa
Alternative Names
LASP-1; LIM and SH3 domain protein 1; LIM and SH3 protein 1; Metastatic lymph node gene 50 protein; MLN 50; MLN50Lasp-1 LASP1 Lasp-1, MLN50 LIM and SH3 protein 1 LIM and SH3 domain protein 1|metastatic lymph node gene 50 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LASP1, check out the LASP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LIM and SH3 domain protein 1 LASP1 Antibody (PA1791)
Hello CJ!
PA1791 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lu C,Fu L,Qian X,Dou L,Cang S.Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.Arch Biochem Biophys.2020 Aug 15;689:108434.doi:10.1016/j.abb.2020.108434.Epub 2020 May 29.PMID:32473899
Species: Human,Mouse
PA1791 usage in article: APP:WB, SAMPLE:HCT116 CELL AND SW480 CELL, DILUTION:NA
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Customer Reviews
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